Sodium chloride (NaCl) solution, especially at a 5 M concentration, is a commonly used stock solution for a variety of solutions. One example of sodium chloride use is to precipitate nucleic acids during their extractions. Show - Download the recipe as a PDF
- 5 M sodium chloride recipe
- How to make a 5 M sodium chloride solution
- Storage of 5 M sodium chloride solution
- Glossary, basic terms to understand...
- Introduction to preparation of solutions.
- Solution 1: Using percentage by weight (w/v)
- Solution 2: Using percentage by volume (v/v)
- Solution 3: Molar Solutions
- 1 Expert Answer
## Download the recipe as a PDFTo download the 5 M sodium chloride recipe as a PDF then click here. ## 5 M sodium chloride recipeThe recipe below can be used to prepare a 100 mL 5 M sodium chloride solution.
## How to make a 5 M sodium chloride solution- Weigh out 29.2 g of sodium chloride and add to a 100 mL Duran bottle.
- Measure out 80 mL of MilliQ water and add to the Duran bottle.
- Dissolve the sodium chloride by adding a magnetic flea into the bottle and placing on a magnetic stirrer. It may take a few minutes to fully dissolve.
- Top up the solution to 100 mL with MilliQ water.
- To sterilise, autoclave the solution on a liquid cycle (20 min at 15 psi).
- Optional: Alternatively, filter the solution using a 0.2 uM filter membrane.
## Storage of 5 M sodium chloride solutionStore 5 M sodium chloride solution at room temperature (+15oC – +25oC). ## SafetySodium chloride is not classified as hazardous. However, always be sure to read the safety data sheet before use.
Lab experiments and types of research often require preparation of chemical solutions in their procedure. We look at preparation of these chemical solutions by weight (w/v) and by volume (v/v). The glossary below cites definitions to know when your work calls for making these and the most accurate molar solutions. To this we add information designed for understanding how to use the pH scale when measuring acidity or alkalinity of a solution. ## Glossary, basic terms to understand...
## Introduction to preparation of solutions.Many experiments involving chemicals call for their use in solution form. That is, two or more substances are mixed together in known quantities. This may involve weighing a precise amount of dry material or measuring a precise amount of liquid. Preparing solutions accurately will improve an experiment's safety and chances for success. ## Solution 1: Using percentage by weight (w/v)## FormulaThe formula for weight percent (w/v) is: [Mass of solute (g) / Volume of solution (ml)] x 100 ## ExampleA 10% NaCl solution has ten grams of sodium chloride dissolved in 100 ml of solution. ## ProcedureWeigh ## Solution 2: Using percentage by volume (v/v)When the solute is a liquid, it is sometimes convenient to express the solution concentration as a volume percent. ## FormulaThe formula for volume percent (v/v) is: [Volume of solute (ml) / Volume of solution (ml)] x 100 ## ExampleMake 1000ml of a 5% by volume solution of ethylene glycol in water. ## Procedure
First, express the percent of solute as a decimal: 5% = 0.05 Multiply this decimal by the total volume: 0.05 x 1000ml = 50ml (ethylene glycol needed). Subtract the volume of solute (ethylene glycol) from the total solution volume: 1000ml (total solution volume) - 50ml (ethylene glycol volume) = 950ml (water needed) Dissolve So, 50ml ethylene glycol / 1000ml solution x100 = 5% (v/v) ethylene glycol solution. ## Solution 3: Molar SolutionsMolar solutions are the most useful in chemical reaction calculations because they directly relate the moles of solute to the volume of solution. ## FormulaThe formula for molarity (M) is: moles of solute / 1 liter of solution or gram-molecular masses of solute / 1 liter of solution. ## Examples
The molecular weight of a sodium chloride molecule (NaCl) is 58.44, so one gram-molecular mass (=1 mole) is 58.44 g. We know this by looking at the periodic table. The atomic mass (or weight) of Na is 22.99, the atomic mass of Cl is 35.45, so 22.99 + 35.45 = 58.44. If you dissolve ## Procedure
To make molar NaCl solutions of other concentrations dilute the mass of salt to 1000ml of solution as follows:
Charity T. asked • 03/05/21
need to dissolve in the solution? ## 1 Expert Answer
Calculate the moles of Sodium chloride required to prepare a solution of molarity 4 and volume 0.5 liters that is 2 moles. Multiply the number of moles with the molar mass of Sodium chloride which is approximately 58.5 g/mo.'' Then your answer is 117 grams. Molarity = moles/liters Moles = Molarity X liters 2 moles of NaCl 58.5 grams NaCl 1 mole NaCl
A 1 molar solution is a solution in which 1 mole of a compound is dissolved in a total volume of 1 litre. For example: The molecular weight of sodium chloride (NaCl) is 58.44, so one gram molecular weight (= 1 mole) is 58.44g. If you dissolve 58.44g of NaCl in a final volume of 1 litre, you have made a 1M NaCl solution. To make a 0.1M NaCl solution, you could weigh 5.844g of NaCl and dissolve it in 1 litre of water; OR 0.5844g of NaCl in 100mL of water (see animation below); OR make a 1:10 dilution of a 1M sample. Making a 0.1M NaCl solution (w/v) Many of the solutions you will use are described in terms of their molarity, so check that you are comforable with the concept by describing how you would make 500mL of a 0.05M NaCl solution.
From Lewis Lab Wiki Jump to navigationJump to searchDissolve 233.76g in 800ml H2O. - Bring volume to 1L.
- Distribute into 10 bottles (100ml each).
- Autoclave on liquid cycle.
## Page 2
From Lewis Lab Wiki Jump to navigationJump to searchYou do not have permission to edit this page, for the following reason: The action you have requested is limited to users in the group: Users. You can view and copy the source of this page. Dissolve 233.76g in 800ml H<sub>2</sub>O. #Bring volume to 1L. #Distribute into 10 bottles (100ml each). #Autoclave on liquid cycle. [[Category:Protocols]] [[Category:Student Worker Solutions]] [[Category:Lab Solutions]]Return to 4M NaCl. ## Page 3From Lewis Lab Wiki
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